Enhanced bone regeneration by Aloe vera gel conjugated barnacle cement protein composite hyaluronic acid hydrogel based hydroxyapatite derived from cuttlefish bone
Author(s):
Dong-Myong Kim, Hyung-Kon Lee, Yong-Seong Kwon, and Yeon-Mea Choi
Injectable Aloe vera gel W (AGW®)-conjugated Barnacle Cement Protein (BCP) composite Hyaluronic Acid (HA) hydrogels provide local periodontal tissue for bone filling in periodontal surgery. We developed a novel type of injectable self-supported hydrogel (2 mg/ml of AGW®-BCP/HA) based cuttlefish bone derived hydroxyapatite (CBH) for dental graft, which could good handling property, biodegradation or biocompatibility with the hydrogel disassembly and provided efficient cell adhesion activity and no inflammatory responses. Herein, the aim of this work was to evaluate bone formation following implantation of CBH and collagen membrane in rabbit calvarias defects. Eight male New Zealand rabbits were used and four circular calvarias defects were created on each animal. Defects were filled with different graft materials: 1) collagen membrane, 2) collagen membrane with CBH, 3) collagen membrane with bovine bone hydroxyapatite (BBH), and 4) control. The animals were sacrificed after 2 and 8 weeks of healing periods for histologic analysis. Both sites receiving CBH and BBH showed statistically increased augmented volume and new bone formation (p<0.05). However, there was no statistical difference in new bone formation between the CBH, BBH and collagen membrane group at all healing periods. Within the limits of this study, collagen membrane with CBH was an effective material for bone formation and space maintaining in rabbit calvarias defects.